Methacarn Fixation for Genomic DNA Analysis in Microdissected, Paraffin-embedded Tissue Specimens
نویسندگان
چکیده
منابع مشابه
Methacarn fixation for genomic DNA analysis in microdissected cells.
We have found methacarn, a non-crosslinking protein-precipitating fixative, to be useful for the analysis of DNA from microdissected specimens of wax-embedded tissue. In this chapter, we present the procedure regarding genomic DNA analysis in methacarn-fixed wax-embedded microdissected rat tissue. Using nested polymerase chain reaction (PCR), and a rapid extraction procedure, fragments of DNA u...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2002
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215540205000911